Micro Pillar Array Columns (µPACTM) Combined with High-Resolution Mass Spectrometry for Skin Ceramide Profiling

Ceramides, a subclass of sphingolipids, are found in all tissues of the human body. The largest diversity is detected in human skin, more specifically in the uppermost layer of the epidermis, i.e. the stratum corneum, were ceramides play a prominent role in the barrier and permeability properties of the skin.

Ceramides are composed of fatty acids conjugated to sphingoid bases via an amide bond. As they display a huge structural diversity, skin ceramide profiling requires a highly efficient chromatographic separation before mass spectrometric measurement. Micro pillar array columns (μPACTM) are perfectly suited in that respect. The inherent high permeability and low ‘on-column’ dispersion obtained by the perfect order of the separation bed makes μPACTM based chromatography unique in its kind.

In this application note, the analysis of strateum corneum samples from healthy volunteers demonstrates its value as inter- and intra-class separation is achieved.