µPACᵀᴹ in practice – The Analytical Scientist, November, 2017
Part I: a case study by our Strategic Advisory Board member Pat Sandra
It is well documented that miniaturization of the separation medium offers several advantages for high-resolution LCMS analysis of complex samples, such as those encountered in proteomics, metabolomics, and biopharmaceutical characterization. The inherent high permeability and low on-column dispersion obtained by the perfect order of the separation bed in micro-pillar array columns (µPAC) is superior to packed beds and monoliths for high-resolution separations. We have recently illustrated this in a number of applications using the first generation of commercially available µPAC to analyze biopharmaceuticals using µPAC combined with mass spectrometry (µPAC-MS), described below. Moreover, the µPAC concept can in the future be tuned and optimized for production of separation media with unique features for high throughput and high productivity applications as well … to follow!
This article is part one of a two-part series, read the other article here