µPACᵀᴹ in practice – The Analytical Scientist, November, 2017
Part II: An interview with Goran Mitulović, Head of the Proteomics Core Facility at the Medical University of Vienna, Austria about his experience with µPACᵀᴹ.
What does μPACᵀᴹ enable you to do differently?
The peak capacity of μPACᵀᴹ columns is significantly higher than the peak capacity of conventional packed columns, leading to a higher number of identifications and, more importantly, better sequence coverage. The low backpressure generated by these columns and the ability to “reverse flush” them add to the benefits. As I mentioned, I use a trap column in front of the μPACᵀᴹ, which removes the majority of possible contaminants; however, when using the same setup with a packed column, I often experienced blockages. So far, the μPACᵀᴹ has been running 24/7 since the beginning of October. There have been no blockages, or pressure increases. Such robustness is surprising!
I’ve also been impressed by the excellent reproducibility, even after repeated injections of extremely complex samples, such as proteins from bone, saliva, urine, serum, and cell cultures.
This article is part of a two-part series, read the first article here