Analytical performance of micro Pillar Array Columns (µPAC) for metabolic profiling


Metabolic profiling studies have become indispensable in a wide range of research fields; including biomarker and drug discovery, toxicology, ‘foodomics’ and plant sciences. Given its tremendous complexity and the dynamic concentration range in which certain metabolites can be present, there is a growing demand for powerful analytical techniques with increased sensitivity to unravel the composition of the metabolome.

Thanks to their proprietary microfluidic design and lithographic manufacturing, μPAC™ nano LC columns can provide unprecedented separation efficiency. This is particularly true in complex analyses where long gradient times are applied to achieve maximal separation.

In this application note, plant extract samples are used to compare the analytical performance of a μPAC™ nano LC column to state-of-the-art UPLC columns. The combination of superior separation performance and increased detection sensitivity will enable analytical scientists to identify even more components in a single metabolic profiling run.