PharmaFluidics_Bottom-Up Proteomic Profiling at Capillary LC Flow Rates_Application Note

Bottom-Up Proteomic Profiling at Capillary LC Flow Rates

In this application note, the performance of PharmaFluidics’ μPAC™ capLC columns is evaluated in a typical bottom-up proteomics workflow. 500 ng of tryptic digest sample (HeLa cell lysate) was injected and separated using reversed phase liquid chromatography over a range of flow rates (2.5-10 μL/min) and solvent gradients (15-60 min). Excellent chromatographic performance could be achieved resulting in an average increase in protein identifications of 20% compared to state-of-the-art packed bed alternatives.