PharmaFluidics_High throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (µPAC™) based capillary-flow LC-MS workflow

High-throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (μPAC™) based capillary-flow LC-MS workflow

In this application note, optimal LC methods for a variety of LC-MS based proteome analysis needs are described. PharmaFluidics’ μPAC™ capLC column is evaluated for different user needs ranging from 12 samples per day in a comprehensive mode and maximizing the data throughput to as high as 65 samples per day for high sample throughput analyses. 2 μg of tryptic digest
sample (HeLa cell lysate) was injected and separated using reversed phase liquid chromatography over a range of flow rates (2-10 μL/min) and solvent gradients (15-120 min).