Next generation non-porous µPAC™ columns for ultra-sensitive nanoLC-MS analysis in limited sample workflows

Next generation non-porous µPAC™ columns for ultra-sensitive nanoLC-MS proteome analysis in limited sample workflows

The performance of the µPAC™ GEN2 Limited Sample Amount column is assessed against classic packed-bed columns for the bottom-up proteomics when working with low amount of sample on an unmodified nano LC-MS instrument.

An increase up to 37% in proteins identification is reported for 10 ng of HeLa digest, resulting from a gain in the identification of the low abundant proteins.

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