Application notes

March 14, 2017

Analytical performance of micro Pillar Array Columns (µPAC) for metabolic profiling

In this application note, plant extract samples are used to compare the analytical performance of a μPAC™ nano LC column to state-of-the-art UPLC columns. The combination of superior separation performance and increased detection sensitivity will enable analytical scientists to identify even more components in a single metabolic profiling run.
June 28, 2017

Peptide separation on micro pillar array columns (µPACTM). Part 1: Cytochrome C tryptic digest

Reversed phase cytochrome c tryptic digest separations are routinely employed for the analysis/quality control of HPLC column performance towards proteomics applications. Easily detectable in UV and with few components, this sample proves to be an ideal tool in demonstrating column performance towards more complex digest separations requiring MS detection. The focus of this application note is to illustrate the performance and versatility of a 200 cm μPAC™ column for peptide separations.
June 28, 2017

Micro Pillar Array Columns (µPACTM) Combined with High-Resolution Mass Spectrometry for Skin Ceramide Profiling

Ceramides, a subclass of sphingolipids, are found in all tissues of the human body. The largest diversity is detected in human skin, more specifically in the uppermost layer of the epidermis, i.e. the stratum corneum, were ceramides play a prominent role in the barrier and permeability properties of the skin. Given the huge structural diversity, skin ceramide profiling requires the use of a highly efficient chromatographic step before mass spectrometric measurement. Micro pillar array columns (μPAC) are perfectly suited in that respect. Their unique benefits are illustrated in this application note.
September 25, 2017

Monoclonal antibody peptide mapping – Remicade® originator and candidate biosimilar

The use of micro Pillar Array Columns (μPACTM) for monoclonal antibody (mAb) peptide mapping is presented. Remicade® (infliximab) originator and candidate biosimilar tryptic digests were analyzed on a 200 cm μPACTM C18 column and peaks eluting were detected by ultraviolet (UV) spectroscopy and high-resolution mass spectrometry (MS). Minute differences such as point mutations in the protein sequence could be detected. μPACTM could thus be of great value in biosimilar characterization.