Evaluation of Micro-Pillar Array Columns (µPAC) Combined with High Resolution Mass Spectrometry for Lipidomics – Koen Sandra et al – LCGC Europe HPLC 2017 Special Issue, Vol. 30, Issue 6, pg 6 – 13.
Obtaining insight in the structure and concentration of the wealth of lipids present in biological samples is of significant value in many areas, including biomarker discovery or drug discovery and development.
The great structural diversity — including also many subtle variations — asks for efficient separation techniques. These should also be able to handle varying physicochemical properties, such as polarity and molecular weight, and the substantial dynamic range of compounds. One milliliter of human plasma, for example, contains lipids at a low femtomole level (eicosanoids) up to micromole level (cholesterol).
In the HPLC 2017 Special issue of LCGC Europe, it is demonstrated how µPACTMcolumns in combination with high-resolution mass spectrometry can tackle these challenges (Vol. 30, Issue 6, pg 6 – 13).
The performance is demonstrated with the analysis of human blood plasma lipids, showing that:
- A high lipidome coverage can be obtained
- Inter- and intra-class separation can be achieved
- Isomeric lipids can be resolved