Of course, we always place our µPAC™ columns inside the column compartment of the HPLC system. Therefore, the temperature is precisely controlled. Similar to other columns, temperature changes will affect analyte retention time. With the µPAC™ column, however, it is not necessary to heat the column to 50°C or 60°C to operate the column at moderate HPLC pump pressures.
Related questionsAt what temperatures can I use the µPAC™ columns?