As the bed volumes for the 50 cm and 200 cm µPAC™ columns are 3 µL and 10 µL, respectively, you need to have sufficient time at the end of your gradient so that all analytes can elute from the µPAC™ column and the column is fully equilibrated before the next sample is injected.

Related media

Technical note

Related questions

What is the optimal flow rate that I should use with a 200 cm µPAC™ column?

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