At BioProcess International 2017 in Boston (USA), the results of a collaboration between the Research Institute for Chromatography and PharmaFluidics were presented. This poster gives an overview of the advantages of µPACTM columns in the characterization of monoclonal antibodies and antibody-drug conjugates (ADC). Herceptin®, Remicade® and Kadcyla® were compared with candidate biosimilars. This illustrates the potential of µPACTM to elucidate post-translational modifications, and other subtle differences such as point mutations. Furthermore, µPACTM allows an in-depth study of conjugation sites and the separation of isomeric conjugated peptides from ADC.