µPACᵀᴹ capLC column

The µPACᵀᴹ capLC column serves those who are looking for increased robustness and throughput without losing sensitivity. 

A flow rate versatility between 1 and 15 µL/min at moderate pressures enables short gradient separations. The µPAC™ technology ensures an exceptionally high reproducibility over time and across laboratories. This makes the µPAC™ capLC column ideally suited for applications such as (clinical) proteomics, metabolomics and biopharmaceutical analyses.

The column is compatible with all commercially available capillary LC systems, and can be integrated smoothly in any experimental set-up.

Must be used in combination with a grounding cable.

This column offers:

Optimal separation performance

Introducing perfect order in the chromatographic separation bed guarantees minimal peak dispersion resulting in high peak capacities and increased proteome coverage. Discover the results in our application note about peptide mapping.

Easy to implement

Plug and play connectivity by introducing commonly used 1/16” fittings from both sides, makes the column compatible with any third party LC vendor.

Extreme robustness

Each column has been manufactured by etching channels and arrays of pillars out of a solid silicon wafer. The column offers a high permeability and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column.

Increased column to column reproducibility

The microfabrication production process, using the same lithographic mask, ensures that every column is identical. Replace µPAC™ capLC columns without shifts in retention time or differences in performance. Obtain results over time and across laboratories, sufficiently consistent for big data analysis.

High flow rate flexibility

The capLC column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates between 1 and 15 µL/min. The entire capillary flow segment is covered with a single column as demonstrated in this webinar.

RELATED APPLICATION NOTES

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PharmaFluidics_High throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (µPAC™) based capillary-flow LC-MS workflow
capLC

High-throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (μPAC™) based capillary-flow LC-MS workflow

In this application note, optimal LC methods for a variety of LC-MS based proteome analysis needs are described. PharmaFluidics’ μPAC™ capLC column is evaluated for …

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PharmaFluidics_Bottom-Up Proteomic Profiling at Capillary LC Flow Rates_Application Note
capLC

Bottom-Up Proteomic Profiling at Capillary LC Flow Rates

In this application note, the performance of PharmaFluidics’ μPAC™ capLC columns is evaluated in a typical bottom-up proteomics workflow. 500 ng of tryptic digest sample …

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RELATED MEDIA

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PharmaFluidics On-demand Webinar_Capillary Flow LC-MS using µPAC™ columns-Combining Sensitivity with Robustness and Throughput_Dr. Geert van Raemdonck_Robert van Ling
Webinar

Webinar – Capillary Flow LC-MS using µPAC™ columns-Combining Sensitivity with Robustness and Throughput

Capillary Flow LC-MS using µPAC™ columns-Combining Sensitivity with Robustness and Throughput Mass-spectrometry based proteomics has become an essential tool in biological, biomedical and biopharmaceutical research. …

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PharmaFluidics On-demand Webinar_A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns_Dr. Geert van Raemdonck_Ali Pervez_
Webinar

Webinar – A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns

A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns In the last decades, several advances have been made in liquid …

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Capillary Flow LCMS Using Micro Pillar Array Columns Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput_Webinar on-Demand_PharmaFluidics_ProGenTomics_
Webinar

Webinar – Capillary Flow LCMS Using micro Pillar Array Columns Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput

In this webcast, Dr. Jeff Op de Beeck of PharmaFluidics will explain the principles of the micro-chip based pillar array columns and demonstrate how the …

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Executive Summary_Capillary Flow LCMS Using Micro Pillar Array Columns Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput_LGGC_PharmaFluidics_ProGenTomics_
Publications

Executive Summary – Capillary Flow LCMS Using Micro Pillar Array Columns Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput

LCGC wrote an executive summary about our webinar with ProGenTomics, Capillary Flow LCMS Using Micro Pillar Array Columns Combining Nano Flow Sensitivity with Analytical Flow …

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Technical notes

Technical note 7: Assessment of capillary flow HPLC column performance

Assessment of capillary flow HPLC column performance In this technical note, the performance of PharmaFluidics µPAC™ capLC columns is compared to the current state-of-the-art in …

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Publications

µPAC™ performance at capillary flow rates

Discover the new µPAC™ CapLC column (1-15 µl/min) – µPAC™ performance at capillary flow rates Unrivalled separation power precise control over the chromatographic bed morphology ensures …

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