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The µPACᵀᴹ capLC column serves those who are looking for increased robustness and throughput without losing sensitivity.
A flow rate versatility between 1 and 15 µL/min at moderate pressures enables short gradient separations. The µPAC™ technology ensures an exceptionally high reproducibility over time and across laboratories. This makes the µPAC™ capLC column ideally suited for applications such as (clinical) proteomics, metabolomics and biopharmaceutical analyses.
The column is compatible with all commercially available capillary LC systems, and can be integrated smoothly in any experimental set-up.
Must be used in combination with a grounding cable.
Introducing perfect order in the chromatographic separation bed guarantees minimal peak dispersion resulting in high peak capacities and increased proteome coverage. Discover the results in our application note about peptide mapping.
Plug and play connectivity by introducing commonly used 1/16” fittings from both sides, makes the column compatible with any third party LC vendor.
Each column has been manufactured by etching channels and arrays of pillars out of a solid silicon wafer. The column offers a high permeability and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column.
The microfabrication production process, using the same lithographic mask, ensures that every column is identical. Replace µPAC™ capLC columns without shifts in retention time or differences in performance. Obtain results over time and across laboratories, sufficiently consistent for big data analysis.
The capLC column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates between 1 and 15 µL/min. The entire capillary flow segment is covered with a single column as demonstrated in this webinar.
The use of micro Pillar Array Columns (μPAC™) for characterizing glycosylation of therapeutic enzymes is presented. Recombinant human acid α-glucosidase (hGAA) was digested and resulting …
In this application note, optimal LC methods for a variety of LC-MS based proteome analysis needs are described. PharmaFluidics’ μPAC™ capLC column is evaluated for …
In this application note, the performance of PharmaFluidics’ μPAC™ capLC columns is evaluated in a typical bottom-up proteomics workflow. 500 ng of tryptic digest sample …
Capillary Flow LC-MS using µPAC™ columns-Combining Sensitivity with Robustness and Throughput Mass-spectrometry based proteomics has become an essential tool in biological, biomedical and biopharmaceutical research. …
Micro Pillar Array Columns for Analysis of Physiological Fluids Micro-pillar arrays provide a highly ordered chromatographic solid phase which minimizes peak dispersion through variability in …
A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns In the last decades, several advances have been made in liquid …
In this webcast, Dr. Jeff Op de Beeck of PharmaFluidics will explain the principles of the micro-chip based pillar array columns and demonstrate how the …
In this webcast, several workflows will be shown. The first part will discuss our collaboration with the researchers from Biognosys, working on a comprehensive proteomics …
In this webcast, Dr. Geert Van Raemdonck of PharmaFluidics will explain the principles of the micro-chip based pillar array columns and demonstrate how the PharmaFluidics …