µPACᵀᴹ Trapping column

The µPACᵀᴹ Trapping column can be effectively used to perform large volume (>5 µL) sample injections on µPACᵀᴹ analytical columns with minimal impact on the total analysis time. Its stationary phase support morphology is designed to perfectly match the analytical µPACᵀᴹ columns, with carefully selected dimensions and surface chemistry.

The trapping column comes in a duo- or five-pack.

This column offers:

Sample preconcentration

The preconcentration of diluted samples or samples with very low peptide content into a sub-microliter trapping volume.

Sample clean-up

An effective removal of salts, reagents and contaminants that have no affinity for hydrophobic bonded phases increasing analytical column lifetime and improving spectral quality and detection sensitivity.

Decreased sample loading times

Depending on the analytical column flow rate, a 10 – 50-fold reduction of the sample loading time for high volume (5 – 20 µL) samples can be achieved.

Extreme robustness

Each trapping column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits.

Flow rate flexibility

The trapping column can be operated at flow rates from 0.1 – 20 µL/min — corresponding column backpressures of up to 40 bar. The maximum operation pressure is 350 bar.

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Technical notes

Technical note 6: Peptide sample enrichment using μPAC™ Trapping columns

Peptide sample enrichment using μPAC™ Trapping columns The online preconcentration of analytes onto low volume trapping columns is a commonly used injection strategy in nano- …

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Videos

Peptide sample enrichment using µPACᵀᴹ Trapping columns

In this video, our  Global Field Support Expert, Geert Van Raemdonck, gives you a quick overview of our technical note ‘Peptide sample enrichment using μPAC™ …

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Webinar

Watch our webinar on demand – Double your IDs for single cell proteomics using micro Pillar Array Columns (µPAC™)

This webinar will explore the potential of newly emerging chromatographic technologies to significantly improve sensitivity for low input proteomics samples, as well as providing detailed …

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