PharmaFluidics - 200cm µPAC™ columns

This column offers:

Optimal separation performance

Obtain more than double the protein identifications than on a 40 cm packed bed column as demonstrated in this webinar.

Improved coverage

µPACᵀᴹ provides comprehensive coverage in single shot proteome analysis, nearing the mark of 10,000 identifications. Discover the results in our executive summary or webinar.

Extreme robustness

Each column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column.

Increased column to column reproducibility

Each column is manufactured using the same lithographic mask, making every column identical. Replace μPACᵀᴹs without retention time shifts. Obtain results over time and across laboratories, sufficiently consistent for big data analysis.

High flow rate flexibility

The column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates (100 – 1,000 nL/min).

RELATED APPLICATION NOTES

  • All
  • Biopharmaceuticals
  • capLC
  • Host cell proteins
  • Limited sample
  • Lipidomics
  • Metabolomics
  • Proteomics
Next generation non-porous µPAC™ columns for ultra-sensitive nanoLC-MS analysis in limited sample workflows
Flex iON Connect

Next generation non-porous µPAC™ columns for ultra-sensitive nanoLC-MS proteome analysis in limited sample workflows

The performance of the µPAC™ GEN2 Limited Sample Amount column is assessed against classic packed-bed columns for the bottom-up proteomics when working with low amount …

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PharmaFluidics_Detailed glycosylation analysis of therapeutic enzymes using µPAC™ capLC-MS and all-ion fragmentation
50cm

Detailed glycosylation analysis of therapeutic enzymes using µPAC™ capLC-MS and all-ion fragmentation

The use of micro Pillar Array Columns (μPAC™) for characterizing glycosylation of therapeutic enzymes is presented. Recombinant human acid α-glucosidase (hGAA) was digested and resulting …

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PharmaFluidics_High throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (µPAC™) based capillary-flow LC-MS workflow
capLC

High-throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (μPAC™) based capillary-flow LC-MS workflow

In this application note, optimal LC methods for a variety of LC-MS based proteome analysis needs are described. PharmaFluidics’ μPAC™ capLC column is evaluated for …

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PharmaFluidics_Bottom-Up Proteomic Profiling at Capillary LC Flow Rates_Application Note
capLC

Bottom-Up Proteomic Profiling at Capillary LC Flow Rates

In this application note, the performance of PharmaFluidics’ μPAC™ capLC columns is evaluated in a typical bottom-up proteomics workflow. 500 ng of tryptic digest sample …

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Host cell proteins

Non-Targeted Host Cell Protein Monitoring in Downstream Process Samples Using Micro Pillar Array Columns Combined with Mass Spectrometry (μPAC™-MS)

Protein biopharmaceuticals have emerged as important therapeutics for the treatment of various diseases including cancer, cardiovascular diseases, diabetes, infection, inflammatory and autoimmune disorders. They come …

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Biopharmaceuticals

Peptide Mapping of Monoclonal Antibodies and Antibody–Drug Conjugates – Using Micro-Pillar Array Columns Combined with Mass Spectrometry

Monoclonal antibodies are becoming a core aspect of the pharmaceutical industry. Together with a huge therapeutic potential, these molecules come with a structural complexity that …

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RELATED MEDIA

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  • Webinar
  • Publications
  • Press releases|News
  • Technical notes
  • News
  • Press releases
  • Events
  • Videos
Technical notes

Technical note 1: perfect order promotes permeability

Perfect order and its role in highly efficient liquid chromatography on micro pillar array columns (µPACTM) – Part 1: Permeability Introduction As stated by Giddings …

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Technical notes

Technical note 2: µPACTM performance in gradient separations

Perfect order and its role in highly efficient liquid chromatography on micro pillar array columns (µPACTM) – Part 2: Performance in gradient separations Introduction As …

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PharmaFluidics_200 cm µPAC™ column
Publications

μPAC™ column technology for single cell proteomics

Read or download the pdf version of this article here. Next to the development of dedicated low-input sample preparation methods, which aim at reducing sample …

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Publications

Evaluation of Micro-Pillar Array Columns (µPACᵀᴹ) Combined with High Resolution Mass Spectrometry for Lipidomics

Evaluation of Micro-Pillar Array Columns (µPACᵀᴹ) Combined with High Resolution Mass Spectrometry for Lipidomics – Koen Sandra et al – LCGC Europe HPLC 2017 Special …

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Publications

PharmaFluidics and ThermoFisher present new proteomics results at HUPO2017

In collaboration with ThermoFisher, PharmaFluidics has presented new results on the benefits of µPAC™ in the analysis of proteomic samples. The poster, presented at HUPO2017, specifically …

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Publications

PharmaFluidics and RIC present data on the comparison of biologicals and candidate biosimilars at BioProcess International 2017

At BioProcess International 2017 in Boston (USA), the results of a collaboration between the Research Institute for Chromatography and PharmaFluidics were presented. This poster gives …

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