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The 200 cm µPACᵀᴹ column provides the ultra-high resolution needed to extract the maximum amount of information from highly complex samples. Dig deeper into the proteome, metabolome or lipidome, or detect minor contaminations or very subtle variations between biomolecules. This is possible by using longer gradients enabled by the benefits of perfect order.
The column is compatible with all commercially available nano-LC systems, and can be integrated smoothly in any experimental set-up.
Must be used in combination with a grounding cable and preferably with a connecting capillary.
Obtain more than double the protein identifications than on a 40 cm packed bed column as demonstrated in this webinar.
µPACᵀᴹ provides comprehensive coverage in single shot proteome analysis, nearing the mark of 10,000 identifications. Discover the results in our executive summary or webinar.
Each column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column.
Each column is manufactured using the same lithographic mask, making every column identical. Replace μPACᵀᴹs without retention time shifts. Obtain results over time and across laboratories, sufficiently consistent for big data analysis.
The column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates (100 – 1,000 nL/min).
The performance of the 50 cm µPAC™ GEN2 Nano column is demonstrated on LCMS instrumentations of different sensitivity levels. The column is also assessed against …
The performance of the µPAC™ GEN2 Limited Sample Amount column is assessed against classic packed-bed columns for the bottom-up proteomics when working with low amount …
The use of micro Pillar Array Columns (μPAC™) for characterizing glycosylation of therapeutic enzymes is presented. Recombinant human acid α-glucosidase (hGAA) was digested and resulting …
In this application note, optimal LC methods for a variety of LC-MS based proteome analysis needs are described. PharmaFluidics’ μPAC™ capLC column is evaluated for …
In this application note, the performance of PharmaFluidics’ μPAC™ capLC columns is evaluated in a typical bottom-up proteomics workflow. 500 ng of tryptic digest sample …
Protein biopharmaceuticals have emerged as important therapeutics for the treatment of various diseases including cancer, cardiovascular diseases, diabetes, infection, inflammatory and autoimmune disorders. They come …
Perfect order and its role in highly efficient liquid chromatography on micro pillar array columns (µPACTM) – Part 1: Permeability Introduction As stated by Giddings …
Perfect order and its role in highly efficient liquid chromatography on micro pillar array columns (µPACTM) – Part 2: Performance in gradient separations Introduction As …
Read or download the pdf version of this article here. Next to the development of dedicated low-input sample preparation methods, which aim at reducing sample …
Evaluation of Micro-Pillar Array Columns (µPACᵀᴹ) Combined with High Resolution Mass Spectrometry for Lipidomics – Koen Sandra et al – LCGC Europe HPLC 2017 Special …
In collaboration with ThermoFisher, PharmaFluidics has presented new results on the benefits of µPAC™ in the analysis of proteomic samples. The poster, presented at HUPO2017, specifically …
At BioProcess International 2017 in Boston (USA), the results of a collaboration between the Research Institute for Chromatography and PharmaFluidics were presented. This poster gives …