This column offers:

Optimal separation performance

Obtain more than double the protein identifications than on a 40 cm packed bed column as demonstrated in this webinar.

Improved coverage

µPACᵀᴹ provides comprehensive coverage in single shot proteome analysis, nearing the mark of 10,000 identifications. Discover the results in our executive summary or webinar.

Extreme robustness

Each column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column.

Increased column to column reproducibility

Each column is manufactured using the same lithographic mask, making every column identical. Replace μPACᵀᴹs without retention time shifts. Obtain results over time and across laboratories, sufficiently consistent for big data analysis.

High flow rate flexibility

The column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates (100 – 1,000 nL/min).


  • All
  • Biopharmaceuticals
  • capLC
  • Host cell proteins
  • Lipidomics
  • Metabolomics
  • Proteomics

Monoclonal antibody peptide mapping – Remicade® originator and candidate biosimilar

The use of micro Pillar Array Columns (μPACᵀᴹ) for monoclonal antibody (mAb) peptide mapping is presented. Remicade® (infliximab) originator and candidate biosimilar tryptic digests were …

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Peptide separation on micro pillar array columns (µPACᵀᴹ). Part 1: Cytochrome C tryptic digest

Reversed phase cytochrome c tryptic digest separations are routinely employed for the analysis/quality control of HPLC column performance towards proteomics applications. Easily detectable in UV …

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Micro Pillar Array Columns (µPACᵀᴹ) Combined with High-Resolution Mass Spectrometry for Skin Ceramide Profiling

Ceramides, a subclass of sphingolipids, are found in all tissues of the human body. The largest diversity is detected in human skin, more specifically in …

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Analytical performance of micro Pillar Array Columns (µPACᵀᴹ) for metabolic profiling

Metabolic profiling studies have become indispensable in a wide range of research fields; including biomarker and drug discovery, toxicology, ‘foodomics’ and plant sciences. Given its …

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PharmaFluidics' Webinar Series_Website

PharmaFluidics’ webinar series

We would like to invite you to join our free webinar series about our µPAC™ technology! During the following weeks we will present five different …

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Webinar_Highly Sensitive Host Cell Protein Analysis_RIC_PharmaFluidics_Koen Sandra_Geert Van Raemdonck-

Webinar – Highly Sensitive Host Cell Protein Analysis using micro Pillar Array Column (µPAC™) LC-MS

Within a rapidly growing biopharmaceutical market, there is an increasing demand for the development of a rising number of biotherapeutic protein drugs, such as monoclonal …

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Webinar: A new LC approach to Complex Biological Samples

Watch this webinar to obtain first hand insight in the ins-and-outs of micro Pillar Array Chromatography (µPACᵀᴹ) columns from our COO, Dr. Paul Jacobs. He …

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Webinar: An innovative nano-LC-MS approach for mAb and ADC characterization

Small structural differences in monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) can have a big impact on biological activity. Revealing every detail of these molecules …

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µPACᵀᴹ in practice – Part II: Goran Mitulović

µPACᵀᴹ in practice – The Analytical Scientist, November, 2017 Part II: An interview with Goran Mitulović, Head of the Proteomics Core Facility at the Medical …

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µPACᵀᴹ in practice – Part I: Pat Sandra

µPACᵀᴹ in practice – The Analytical Scientist, November, 2017 Part I: a case study by our Strategic Advisory Board member Pat Sandra It is well …

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We're updating our column format!

To be able to keep up with demand, we’ve upscaled our production. In this process, we’ve switched to a longer and thinner casing format, allowing the columns to fit in a larger range of column ovens. The physical properties and chromatographic performance remain unchanged.