This column offers:

Optimal separation performance

Obtain more than double the protein identifications than on a 40 cm packed bed column as demonstrated in this webinar.

Improved coverage

µPACᵀᴹ provides comprehensive coverage in single shot proteome analysis, nearing the mark of 10,000 identifications. Discover the results in our executive summary or webinar.

Extreme robustness

Each column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column.

Increased column to column reproducibility

Each column is manufactured using the same lithographic mask, making every column identical. Replace μPACᵀᴹs without retention time shifts. Obtain results over time and across laboratories, sufficiently consistent for big data analysis.

High flow rate flexibility

The column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates (100 – 1,000 nL/min).

RELATED APPLICATION NOTES

  • All
  • Biopharmaceuticals
  • capLC
  • Host cell proteins
  • Lipidomics
  • Metabolomics
  • Proteomics
Biopharmaceuticals

Monoclonal antibody peptide mapping – Herceptine® originator and candidate biosimilar

The use of micro Pillar Array Columns (μPACᵀᴹ) for monoclonal antibody (mAb) peptide mapping is presented. Herceptine® (trastuzumab) originator and candidate biosimilar tryptic digests were …

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Biopharmaceuticals

Monoclonal antibody peptide mapping – Remicade® originator and candidate biosimilar

The use of micro Pillar Array Columns (μPACᵀᴹ) for monoclonal antibody (mAb) peptide mapping is presented. Remicade® (infliximab) originator and candidate biosimilar tryptic digests were …

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Proteomics

Peptide separation on micro pillar array columns (µPACᵀᴹ). Part 1: Cytochrome C tryptic digest

Reversed phase cytochrome c tryptic digest separations are routinely employed for the analysis/quality control of HPLC column performance towards proteomics applications. Easily detectable in UV …

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Lipidomics

Micro Pillar Array Columns (µPACᵀᴹ) Combined with High-Resolution Mass Spectrometry for Skin Ceramide Profiling

Ceramides, a subclass of sphingolipids, are found in all tissues of the human body. The largest diversity is detected in human skin, more specifically in …

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Metabolomics

Analytical performance of micro Pillar Array Columns (µPACᵀᴹ) for metabolic profiling

Metabolic profiling studies have become indispensable in a wide range of research fields; including biomarker and drug discovery, toxicology, ‘foodomics’ and plant sciences. Given its …

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RELATED MEDIA

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  • Webinar
  • Publications
  • Press releases|News
  • Technical notes
  • News
  • Press releases
  • Events
  • Videos
Publications

New format for PharmaFluidics’ μPAC™ columns to meet surging customer demand

Over the recent months, PharmaFluidics has focused on scaling up production of its μPAC™ nano-LC columns in response to a surge in customer demand and …

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Webinar

PharmaFluidics’ webinar series

Did you miss one of our webinars about our µPAC™ technology? Watch them on-demand below! A New LC Approach to Complex Biological Samples by using …

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PharmaFluidics On-demand Webinar_A Novel Nanoflow LC–MS Approach for Bottom-up Proteomics Using the 200 cm long µPAC™ column_Dr. Geert van Raemdonck_Gilles Jaouen
Webinar

Webinar – A Novel Nanoflow LC–MS Approach for Bottom-up Proteomics Using the 200 cm long µPAC™ column

A Novel Nanoflow LC–MS Approach for Bottom-up Proteomics Using the 200 cm long µPAC™ column Bottom-up proteomics using 50 to 100 µm C18 packed capillaries …

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PharmaFluidics On-demand Webinar_A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns_Dr. Geert van Raemdonck_Ali Pervez_
Webinar

Webinar – A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns

A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns In the last decades, several advances have been made in liquid …

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Webinar_Highly Sensitive Host Cell Protein Analysis_RIC_PharmaFluidics_Koen Sandra_Geert Van Raemdonck-
Webinar

Webinar – Highly Sensitive Host Cell Protein Analysis using micro Pillar Array Column (µPAC™) LC-MS

Within a rapidly growing biopharmaceutical market, there is an increasing demand for the development of a rising number of biotherapeutic protein drugs, such as monoclonal …

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Webinar

Webinar: A new LC approach to Complex Biological Samples

Watch this webinar to obtain first hand insight in the ins-and-outs of micro Pillar Array Chromatography (µPACᵀᴹ) columns from our COO, Dr. Paul Jacobs. He …

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