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The 200 cm µPACᵀᴹ column provides the ultra-high resolution needed to extract the maximum amount of information from highly complex samples. Dig deeper into the proteome, metabolome or lipidome, or detect minor contaminations or very subtle variations between biomolecules. This is possible by using longer gradients enabled by the benefits of perfect order.
The column is compatible with all commercially available nano-LC systems, and can be integrated smoothly in any experimental set-up.
Must be used in combination with a grounding cable and preferably with a connecting capillary.
Obtain more than double the protein identifications than on a 40 cm packed bed column as demonstrated in this webinar.
µPACᵀᴹ provides comprehensive coverage in single shot proteome analysis, nearing the mark of 10,000 identifications. Discover the results in our executive summary or webinar.
Each column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column.
Each column is manufactured using the same lithographic mask, making every column identical. Replace μPACᵀᴹs without retention time shifts. Obtain results over time and across laboratories, sufficiently consistent for big data analysis.
The column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates (100 – 1,000 nL/min).
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In a recent article in the March edition LCGC Dr. Koen Sandra is showing new data on the resolving power of the µPAC™ column for …
Watch this webinar to hear, Dr. Jeff Op de Beeck, the Application Development Manager of PharmaFluidics, explain theµPAC™ technology. He is joined by Dr. Francis …
μPAC™ column robustness in bottom-up proteomics Next to technical issues that can occur with the LC-MS system’s hard- and software, LC column failure is one …
Watch this webinar to hear, Dr. Jeff Op de Beeck, the Application Development Manager of PharmaFluidics, explain the µPAC™ technology and Dr. Goran Mitulović, head …
LCGC wrote an executive summary about our webinar, A Novel Nanoflow LC-MS Approach for Bottom-up Proteomics Using Micro Pillar Array Columns. Our Application Development Manager, …
μPAC™ column performance with peptide standards: Thermo Scientific™ Pierce™ retention time calibration mixture Peptide standards are critical in mass spectrometry based proteomics to ensure optimal …
To be able to keep up with demand, we’ve upscaled our production. In this process, we’ve switched to a longer and thinner casing format, allowing the columns to fit in a larger range of column ovens. The physical properties and chromatographic performance remain unchanged.