This column offers:

Optimal separation performance

Obtain more than double the protein identifications than on a 40 cm packed bed column as demonstrated in this webinar.

Improved coverage

µPACᵀᴹ provides comprehensive coverage in single shot proteome analysis, nearing the mark of 10,000 identifications. Discover the results in our executive summary or webinar.

Extreme robustness

Each column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column.

Increased column to column reproducibility

Each column is manufactured using the same lithographic mask, making every column identical. Replace μPACᵀᴹs without retention time shifts. Obtain results over time and across laboratories, sufficiently consistent for big data analysis.

High flow rate flexibility

The column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates (100 – 1,000 nL/min).

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Publications

Executive Summary LCGC – Expanding the Limits of Shotgun Proteomics Using Micro-Pillar Array Columns and Data-Independent Acquisition Methods

LCGC wrote an executive summary about our webinar with Biognosys, Expanding the Limits of Shotgun Proteomics Using Micro-Pillar Array Columns and Data-Independent Acquisition Methods. Our …

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PharmaFluidics_μPAC™ Flex iON Connect
Technical notes

Technical note 9: µPAC™ Flex iON Connect ESI-MS interface

µPAC™ Flex iON Connect ESI-MS interface The μPAC™ Flex iON Connect is a user-friendly interface to facilitate fast, reproducible and low dispersion connection of μPAC™ …

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Technical note 8_µPAC™ LC, a new era in column-to-column reproducibility
Technical notes

Technical note 8: µPAC™ LC, a new era in column-to-column reproducibility

µPAC™ LC, a new era in column-to-column reproducibility The main goal of this study is to compare the column-to-column reproducibility of the μPAC™ capLC column …

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Publications

Micro Pillar Array Column Technology Coupled to PASEF: A Sensitive Approach to Analyze Host Cell Proteins

Improve your detection of Host Cell Proteins (HCPs) in monoclonal antibody (mAb) preparations by implementing a workflow combination with 200cm µPAC™ C18 columns, parallel accumulation …

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Publications

μPAC™ Selection Tree

This µPAC™ installation flowchart will guide you through the installation of the µPAC™ columns on the most commonly used LC-MS configurations. Download here Select the …

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Publications

Koen Sandra – Overcoming antibody analytical challenges using the µPAC™ column

Biopharmaceutical products, such as monoclonal antibodies, are on the rise! Biopharmaceuticals currently account for 25% of the total pharmaceutical market and monoclonal antibodies are the …

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