This column offers:

Increased separation performance

More than double the number of peptides and 72% more proteins identified in a 60 min analysis compared to a 15 cm packed bed column. Find out more in this technical note.

Improved coverage

The ultra-sensitive analysis of low-input proteomics samples enabled twice as many unique peptide and protein groups identifications compared to a traditional column. Discover the results in our webinar.

Extreme robustness

Each column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column.

Increased column to column reproducibility

Each column is manufactured using the same lithographic mask, making every column identical. Replace µPACᵀᴹs with minimal impact. Obtain results over time and across laboratories, sufficiently consistent for big data analysis.

High flow rate flexibility

The column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates (100 – 2,000 nL/min). The maximum operating pressure is 350 bar.

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Technical notes

Technical note 5: Routine proteome analysis using 50 cm μPAC™ columns

Routine proteome analysis using 50 cm μPAC™ columns The practice of bottom-up proteomics relies to a large extent on the separation performance that can be …

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PharmaFluidics_μPAC™ Flex iON Connect
Technical notes

Technical note 9: µPAC™ Flex iON Connect ESI-MS interface

µPAC™ Flex iON Connect ESI-MS interface The μPAC™ Flex iON Connect is a user-friendly interface to facilitate fast, reproducible and low dispersion connection of μPAC™ …

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Technical note 8_µPAC™ LC, a new era in column-to-column reproducibility
Technical notes

Technical note 8: µPAC™ LC, a new era in column-to-column reproducibility

µPAC™ LC, a new era in column-to-column reproducibility The main goal of this study is to compare the column-to-column reproducibility of the μPAC™ capLC column …

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Webinar

Watch our webinar on demand – Double your IDs for single cell proteomics using micro Pillar Array Columns (µPAC™)

This webinar will explore the potential of newly emerging chromatographic technologies to significantly improve sensitivity for low input proteomics samples, as well as providing detailed …

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Publications

Routine Proteome Analysis Using 50 cm micro Pillar Array Columns – GEN

According to PharmaFluidics, more sample components can be identified if separation channels are etched in silicon. The practice of bottom-up proteomics relies to a large …

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Publications

μPAC™ Selection Tree

This µPAC™ installation flowchart will guide you through the installation of the µPAC™ columns on the most commonly used LC-MS configurations. Download here Select the …

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