Technical note 2: µPACTM performance in gradient separations

Perfect order and its role in highly efficient liquid chromatography on micro pillar array columns (µPACTM) – Part 2: Performance in gradient separations

Introduction

As discussed in part one of this technical note, the perfect order of μPACTM stationary phases results in reduced peak dispersion and low column backpressure. It was shown that a μPACTM cartridge with a separation length of 200 cm produces up to 300.000 plates (k’=2) and can be operated in a wide range of flow rates (up to 1,500 nl/min) while keeping the pressure under control (< 350 bar). In this respect, μPACsTM considerably outperform conventional nano LC columns.

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