Technical note 8_µPAC™ LC, a new era in column-to-column reproducibility

Technical note 8: µPAC™ LC, a new era in column-to-column reproducibility

µPAC™ LC, a new era in column-to-column reproducibility

The main goal of this study is to compare the column-to-column reproducibility of the μPAC™ capLC column format to state-of-the art commercially available packed bed column alternatives. The basic performance characteristics of the μPAC™ capLC have already been discussed in previous technical and application notes*. Reversed phase capillary LC analysis of a protein tryptic digest (Cytochrome C digest) is performed on a series of columns (three column types, n=3), and column-to-column reproducibility is compared in terms of retention, efficiency and peak shape.

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* Read the previous technical and application notes
Technical note 7: Assessment of capillary flow HPLC column performance
Bottom-Up Proteomic Profiling at Capillary LC Flow Rates
High-throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (μPAC™) based capillary-flow LC-MS workflow