µPAC in practice – The Analytical Scientist, November, 2017
Part I: a case study by our Strategic Advisory Board member Pat Sandra
Part II: An interview with Goran Mitulović, Head of the Proteomics Core Facility at the Medical University of Vienna, Austria about his experience with µPAC.
What does μPAC enable you to do differently?
The peak capacity of μPAC columns is significantly higher than the peak capacity of conventional packed columns, leading to a higher number of identifications and, more importantly, better sequence coverage. The low backpressure generated by these columns and the ability to “reverse flush” them add to the benefits. As I mentioned, I use a trap column in front of the μPAC, which removes the majority of possible contaminants; however, when using the same setup with a packed column, I often experienced blockages. So far, the μPAC has been running 24/7 since the beginning of October. There have been no blockages, or pressure increases. Such robustness is surprising!
I’ve also been impressed by the excellent reproducibility, even after repeated injections of extremely complex samples, such as proteins from bone, saliva, urine, serum, and cell cultures.