Webinar: An innovative nano-LC-MS approach for mAb and ADC characterization

Small structural differences in monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) can have a big impact on biological activity. Revealing every detail of these molecules is very challenging, but essential in the production and approval of new compounds or biosimilars.

In this webcast, Dr. Paul Jacobs of PharmaFluidics explains the principles and robustness of the micro-chip based pillar array columns. Dr. Koen Sandra of the Research Institute of Chromatography (RIC) demonstrates how micro pillar array columns, in combination with ultraviolet (UV) spectroscopy and high-resolution mass spectrometry (MS), can be a very powerful tool to characterize mAbs and investigate the comparability between originator and biosimilar mAbs and to identify antibody-drug conjugation sites.


  • Learn the principles of micro-chip based Pillar Array Chromatography columns
  • Understand the benefits and separation performance of micro-chip based chromatography columns for the analysis of complex biopharmaceutical samples
  • See examples of mAb peptide mapping and the characterization of ADCs
  • Get the proof of how the micro-chip based chromatography columns can be applied in the analytical workflow