Tips and tricks when working with PharmaFluidics’ micro Pillar Array Columns
Compared to traditional LC columns, PharmaFluidics uses a fundamentally different approach to fabricate their chromatographic columns. Instead of using particles that are packed into a tube or capillary, the stationary phase is designed in a similar way that electronic circuits or sensors are designed using state-of-the-art lithographic techniques to transfer these designs on silicon wafers. Microfluidic channels are created on a silicon wafer by etching into the silicon. These channels are filled with accurately positioned ordered arrays of pillars, which form the backbone of the stationary phase. The result is a top performing LC column that is very robust and is much less prone to sample related column failure.
Although these columns can easily be exchanged with conventional alternatives, we want to provide you some tips and tricks regarding good practices to get the best performance out of the µPAC™ columns. First of all we would like to discuss some points of interest regarding the installation and connections on the LC system. Further we would like to review the interface between column and ESI source to get the most out of your µPAC™ column. Finally, we also want to provide some recommendations regarding the most optimal LC gradients and conditions that can be used for specific applications. All topics will be supported by examples and illustrations as much as possible.
In this webinar Dr. Geert van Raemdonck and Dr. Keiryn L. Bennett of PharmaFluidics provided some tips and tricks when working with micro-chip based pillar array columns and discussed the most optimal configuration when using micro-chip based pillar array columns.